You are not currently logged in.
Access JSTOR through your library or other institution:
If You Use a Screen ReaderThis content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Effects of Simulated Microgravity on the Development and Maturation of Dissociated Cortical Neurons
Alessio Crestini, Cristina Zona, Pierluigi Sebastiani, Massimo Pieri, Valentina Caracciolo, Lorenzo Malvezzi-Campeggi, Annamaria Confaloni and Silvia Di Loreto
In Vitro Cellular & Developmental Biology. Animal
Vol. 40, No. 5/6 (May - Jun., 2004), pp. 159-165
Published by: Society for In Vitro Biology
Stable URL: http://www.jstor.org/stable/4295545
Page Count: 7
You can always find the topics here!Topics: Neurons, Microgravity, Simulations, Cultured cells, Neuroglia, Cell culture techniques, Reactive oxygen species, Cell aggregates, Fluorescence, Astrocytes
Were these topics helpful?See somethings inaccurate? Let us know!
Select the topics that are inaccurate.
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Preview not available
Although a wealth of evidence supports the hypothesis that some functions of the nervous system may be altered during exposure to microgravity, the possible changes in basic neuronal physiology are not easy to assess. Indeed, few studies have examined whether microgravity affects the development of neurons in culture. In the present study, a suspension of dissociated cortical cells from rat embryos were exposed to 24 h of simulated microgravity before plating in a normal adherent culture system. Both preexposed and control cells were used after a period of 7-10 d in vitro. The vitality and the level of reactive oxygen species of cultures previously exposed did not differ from those of normal cultures. Cellular characterization by immunostaining with a specific antibody displayed normal neuronal phenotype in control cells, whereas pretreatment in simulated microgravity revealed an increase of glial fibrillary acidic protein fluorescence in the elongated stellate glial cells. Electrophysiological recording indicated that the electrical properties of neurons preexposed were comparable with those of controls. Overall, our results indicate that a short time of simulated microgravity preexposure does not affect dramatically the ability of dissociated neural cells to develop and differentiate in an adherent culture system.
In Vitro Cellular & Developmental Biology. Animal © 2004 Society for In Vitro Biology