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Maintenance of Embedded Pig Pancreatic Pseudo-Islets in a Collagen Gel Matrix: Study of the Effect of Hydrocortisone, a Collagenase Inhibitor, and Nicotinamide on Collagenolysis and the Morphogenesis of Pancreatic Islet-Cells in Collagen Gel Matrix

Hisako Ohgawara, Naomi Mochizuki, Toshio Taira, Sachiyo Nishijima, Naoko Iwasaki, Ryougo Yui, Yukimasa Hirata and Taro Hayakawa
In Vitro Cellular & Developmental Biology
Vol. 26, No. 4 (Apr., 1990), pp. 348-352
Stable URL: http://www.jstor.org/stable/4296446
Page Count: 5
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Maintenance of Embedded Pig Pancreatic Pseudo-Islets in a Collagen Gel Matrix: Study of the Effect of Hydrocortisone, a Collagenase Inhibitor, and Nicotinamide on Collagenolysis and the Morphogenesis of Pancreatic Islet-Cells in Collagen Gel Matrix
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Abstract

We describe a method for maintaining neonatal pig pancreatic isletlike cell clusters (as pseudo-islets) embedded in a collagen gel matrix for long periods. The pseudo-islets were formed from single cells of pig pancreas maintained in a suspension culture and then embedded in pepsin-solubilized type I collagen. When the pseudo-islets were cultured in the collagen matrix, the amount of collagen in the culture decreased gradually during the culture period as soluble hydroxyproline-containing material accumulated in the medium. A low concentration of collagen (0.16%) degraded the collagen gels more rapidly than did high concentrations of collagen (0.64%). The degradation of collagen depended both on the number of pseudo-islets embedded in the gel matrix and on the culture conditions used to maintain them. With added nicotinamide, the accumulation of hydroxyproline decreased in the medium and the structure of the gel matrix was well maintained. Hydrocortisone or a specific inhibitor of collagenase did not decrease the solubilization of embedded pseudo-islet cultures and did not help to maintain their structure. These observations indicate the possible utility of long-term maintenance of pseudo-islets in collagen gel matrix in the presence of nicotinamide.

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