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A Serum-Free Medium for the Culture of Insect Cells and Production of Recombinant Proteins
W. F. Hink
In Vitro Cellular & Developmental Biology
Vol. 27A, No. 5 (May, 1991), pp. 397-401
Published by: Society for In Vitro Biology
Stable URL: http://www.jstor.org/stable/4296672
Page Count: 5
You can always find the topics here!Topics: Lipids, Cultured cells, Cell growth, Cell lines, Viability, Flasks, Insect vectors, Recombinant proteins, Baculoviridae, Insect genetics
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A low protein aqueous lipid supplement (Ex-Cyte VLE), in combination with pluronic polyol, is an effective replacement for fetal bovine serum for insect Sf-9 cells. Serum-free medium with lipid supplement and pluronic (SFM-LP) supported higher cell viability and maximum cell populations than serum-supplemented medium. No adaptation procedures are required when switching cells from serum-containing medium to SFM-LP, and growth rates remain constant during continued passages in SFM-LP. The amounts of recombinant proteins produced, which is the major use for the Sf-9 cells, are better or equal in SFM-LP compared to serum-supplemented medium. SFM-LP also supports growth of the TN-368 cell line but IPLB-SF-21AE or IZD-Mb0503 lines grow poorly in this medium.
In Vitro Cellular & Developmental Biology © 1991 Society for In Vitro Biology