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α-Smooth Muscle Actin Expression in Cultured Cardiac Fibroblasts of Newborn Rat

Daniele Brouty-Boye, Despina Kolonias, Niramol Savaraj and Theodore J. Lampidis
In Vitro Cellular & Developmental Biology
Vol. 28A, No. 4 (Apr., 1992), pp. 293-296
Stable URL: http://www.jstor.org/stable/4296850
Page Count: 4
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α-Smooth Muscle Actin Expression in Cultured Cardiac Fibroblasts of Newborn Rat
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Abstract

Using a panel of monoclonal anitbodies to several different cytoskeletal elements in primary cultures derived from newborn rat hearts we report that fibroblasts similar to cardiac-muscle cells expressed the α-actin isoform of smooth muscle cells. However, striated muscle α-actin or desmin antibodies did not stain cardiac fibroblasts but did stain cardiac-muscle cells. The α-smooth muscle actin distributed as a stress fiber and in a cross-striated pattern in cardiac muscle while fibroblasts showed exclusive stress fiber staining. These results suggest that connective tissue cells during development of the heart contain muscle-specific elements which may relate to the organ-specific contractile function with which they are associated.

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