Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Prp43: An RNA Helicase-Like Factor Involved in Spliceosome Disassembly

Jaime E. Arenas and John N. Abelson
Proceedings of the National Academy of Sciences of the United States of America
Vol. 94, No. 22 (Oct. 28, 1997), pp. 11798-11802
Stable URL: http://www.jstor.org/stable/42972
Page Count: 5
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Prp43: An RNA Helicase-Like Factor Involved in Spliceosome Disassembly
Preview not available

Abstract

The Saccharomyces cerevisiae genes PRP2, PRP16, and PRP22 encode pre-mRNA splicing factors that belong to the highly conserved ``DEAH'' family of putative RNA helicases. We previously identified two additional members of this family, JA1 and JA2. To investigate its biological function, we cloned the JA1 gene and generated alleles carrying mutations identical to those found in highly conserved regions of other members of the DEAH family. A ja1 allele carrying a mutation identical to that in the temperature-sensitive (ts) prp22-1 gene conferred ts phenotype when integrated into the genome of a wild-type strain by gene replacement. Northern analysis of RNA obtained from the ts strain shifted to a nonpermissive temperature revealed accumulation of unspliced pre-mRNAs and excised intron lariats. Furthermore, analysis of splicing complexes showed that intron lariats accumulated in spliceosomes. The results presented indicate that JA1 encodes a pre-mRNA processing factor (Prp) involved in disassembly of spliceosomes after the release of mature mRNA. We have therefore renamed this gene PRP43.

Page Thumbnails

  • Thumbnail: Page 
11798
    11798
  • Thumbnail: Page 
11799
    11799
  • Thumbnail: Page 
11800
    11800
  • Thumbnail: Page 
11801
    11801
  • Thumbnail: Page 
11802
    11802