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DNA Bending and the Initiation of Transcription at σ 54-Dependent Bacterial Promoters

Manuel Carmona, Felix Claverie-Martin and Boris Magasanik
Proceedings of the National Academy of Sciences of the United States of America
Vol. 94, No. 18 (Sep. 2, 1997), pp. 9568-9572
Stable URL: http://www.jstor.org/stable/43064
Page Count: 5
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
DNA Bending and the Initiation of Transcription at σ 54-Dependent Bacterial Promoters
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Abstract

We have examined the effects on transcription initiation of promoter and enhancer strength and of the curvature of the DNA separating these entities on wild-type and mutated enhancer-promoter regions at the Escherichia coli σ 54-dependent promoters glnAp2 and glnHp2 on supercoiled and linear DNA. Our results, together with previously reported observations by other investigators, show that the initiation of transcription on linear DNA requires a single intrinsic or induced bend in the DNA, as well as a promoter with high affinity for σ 54-RNA polymerase, but on supercoiled DNA requires either such a bend or a high affinity promoter but not both. The examination of the DNA sequence of all nif gene activator- or nitrogen regulator I-σ 54 promoters reveals that those lacking a binding site for the integration host factor have an intrinsic single bend in the DNA separating enhancer from promoter.

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