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INHERITANCE OF ISOZYME PHENOTYPES OF PINUS MERKUSII

S. Changtragoon and R. Finkeldey
Journal of Tropical Forest Science
Vol. 8, No. 2 (December 1995), pp. 167-177
Stable URL: http://www.jstor.org/stable/43582474
Page Count: 11
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
INHERITANCE OF ISOZYME PHENOTYPES OF PINUS MERKUSII
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Abstract

Isozyme analysis is a powerful tool for studying the population genetics of forest trees. The identification of isozyme marker gene loci is based on a genetic analysis, i.e. the proof of the genetic control and a determination of the mode of inheritance of trait expressions (isozyme phenotypes). The observation of segregation ratios within the haploid endosperms of putatively heterozygous seed trees and their comparison to the expected 1:1 ratio of the two trait expressions is a simple and straightforward method of genetic analysis for most conifer species. Using this approach, 11 polymorphic isozyme gene loci were identified for Pinus merkusii, a Southeast Asian pine. Another 7 putative gene loci were found to be completely or nearly completely monomorphic for all populations investigated, rendering a genetic analysis impracticable. Analisis isozim merupakan satu alat berkesan untuk mengkaji genetik populasi pokok-pokok hutan. Pengecaman lokus-lokus gen penanda isozim adalah berdasarkan analisis genetik, iaitu bukti kawalan genetik dan penentuan mod pewarisan ciri-ciri ekspresi (fenotip isozim). Pemerhatian nisbah-nisbah pengasingan dalam endosperma haploid bagi pokok-pokok berbiji he terozigus dan putatif serta perbandingan dengan nisbah jangkaan 1:1 bagi kedua-dua ciri ekspresi merupakan satu cara yang mudah untuk menjalankan analisis genetik bagi kebanyakan spesies konifer. Dengan menggunakan cara ini, 11 lokus gen isozim polimorfik telah dikenalpasti bagi Pinus merkusii, sejenis pain Asia Tenggara. Tujuh lagi lokus gen putatif didapati monomorfik sepenuhnya atau hampir monomorfik sepenuhnya bagi semua populasi yang dikaji, mengakibatkan análisis genetik tidak praktikal.

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