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Targeted Deletion of Smad4 Shows it is Required for Transforming Growth Factor β and Activin Signaling in Colorectal Cancer Cells
Shibin Zhou, Phillip Buckhaults, Leigh Zawel, Fred Bunz, Greg Riggins, Jia Le Dai, Scott E. Kern, Kenneth W. Kinzler and Bert Vogelstein
Proceedings of the National Academy of Sciences of the United States of America
Vol. 95, No. 5 (Mar. 3, 1998), pp. 2412-2416
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/44064
Page Count: 5
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Smad4 (DPC4) is a candidate tumor suppressor gene that has been hypothesized to be critical for transmitting signals from transforming growth factor (TGF) β and related ligands. To directly test this hypothesis, the Smad4 gene was deleted through homologous recombination in human colorectal cancer cells. This deletion abrogated signaling from TFG-β as well as from the TGF-β family member activin. These results provide unequivocal evidence that mutational inactivation of Smad4 causes TGF-β unresponsiveness and provide a basis for understanding the physiologic role of this gene in tumorigenesis.
Proceedings of the National Academy of Sciences of the United States of America © 1998 National Academy of Sciences