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A Selective Medium for the Cultivation of N. gonorrhoeae and N. meningitidis

James D. Thayer and John E. Martin, Jr.
Public Health Reports (1896-1970)
Vol. 79, No. 1 (Jan., 1964), pp. 49-57
Published by: Sage Publications, Inc.
DOI: 10.2307/4592051
Stable URL: http://www.jstor.org/stable/4592051
Page Count: 9
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A Selective Medium for the Cultivation of N. gonorrhoeae and N. meningitidis
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Abstract

A medium with selective properties which permit growth of gonococci and meningococci while inhibiting most other Neisseria species was prepared by adding polymyxin B (25 units per milliliter) and ristocetin (10 micrograms per milliliter) to conventional diagnostic media. Overgrowth of gonococcal colonies by bacterial contaminants encountered in cervical, vaginal, and rectal specimens was almost totally prevented, and the sometimes diagnostically confusing Mima polymorpha var. oxidans failed to grow.

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