Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

MSF (MLL Septin-Like Fusion), a Fusion Partner Gene of MLL, in a Therapy-Related Acute Myeloid Leukemia with a t(11;17)(q23;q25)

Mitsuhiko Osaka, Janet D. Rowley and Nancy J. Zeleznik-Le
Proceedings of the National Academy of Sciences of the United States of America
Vol. 96, No. 11 (May 25, 1999), pp. 6428-6433
Stable URL: http://www.jstor.org/stable/47887
Page Count: 6
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
MSF (MLL Septin-Like Fusion), a Fusion Partner Gene of MLL, in a Therapy-Related Acute Myeloid Leukemia with a t(11;17)(q23;q25)
Preview not available

Abstract

MLL (ALL1, Htrx, HRX), which is located on chromosome band 11q23, frequently is rearranged in patients with therapy-related acute myeloid leukemia who previously were treated with DNA topoisomerase II inhibitors. In this study, we have identified a fusion partner of MLL in a 10-year-old female who developed therapy-related acute myeloid leukemia 17 months after treatment for Hodgkin's disease. Leukemia cells of this patient had a t(11;17)(q23;q25), which involved MLL as demonstrated by Southern blot analysis. The partner gene was cloned from cDNA of the leukemia cells by use of a combination of adapter reverse transcriptase-PCR, rapid amplification of 5 cDNA ends, and BLAST database analysis to identify expressed sequence tags. The full-length cDNA of 2.8 kb was found to be an additional member of the septin family, therefore it was named MSF (MLL septin-like fusion). Members of the septin family conserve the GTP binding domain, localize in the cytoplasm, and interact with cytoskeletal filaments. A major 4-kb transcript of MSF was expressed ubiquitously; a 1.7-kb transcript was found in most tissues. An additional 3-kb transcript was found only in hematopoietic tissues. By amplification with MLL exon 5 forward primer and reverse primers in MSF, the appropriately sized products were obtained. MSF is highly homologous to hCDCrel-1, which is a partner gene of MLL in leukemias with a t(11;22)(q23;q11.2). Further analysis of MSF may help to delineate the function of MLL partner genes in leukemia, particularly in therapy-related leukemia.

Page Thumbnails

  • Thumbnail: Page 
6428
    6428
  • Thumbnail: Page 
6429
    6429
  • Thumbnail: Page 
6430
    6430
  • Thumbnail: Page 
6431
    6431
  • Thumbnail: Page 
6432
    6432
  • Thumbnail: Page 
6433
    6433