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Interaction between Astrocytes and Adult Subventricular Zone Precursors Stimulates Neurogenesis
Daniel A. Lim and Arturo Alvarez-Buylla
Proceedings of the National Academy of Sciences of the United States of America
Vol. 96, No. 13 (Jun. 22, 1999), pp. 7526-7531
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/48092
Page Count: 6
You can always find the topics here!Topics: Astrocytes, B lymphocytes, Neurons, Neural stem cells, Neurogenesis, Cells, Neuroglia, Cultured cells, Coculture techniques, Stem cells
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Neurogenesis continues in the mammalian subventricular zone (SVZ) throughout life. However, the signaling and cell-cell interactions required for adult SVZ neurogenesis are not known. In vivo, migratory neuroblasts (type A cells) and putative precursors (type C cells) are in intimate contact with astrocytes (type B cells). Type B cells also contact each other. We reconstituted SVZ cell-cell interactions in a culture system free of serum or exogenous growth factors. Culturing dissociated postnatal or adult SVZ cells on astrocyte monolayers--but not other substrates--supported extensive neurogenesis similar to that observed in vivo. SVZ precursors proliferated rapidly on astrocytes to form colonies containing up to 100 type A neuroblasts. By fractionating the SVZ cell dissociates with differential adhesion to immobilized polylysine, we show that neuronal colony-forming precursors were concentrated in a fraction enriched for type B and C cells. Pure type A cells could migrate in chains but did not give rise to neuronal colonies. Because astrocyte-conditioned medium alone was not sufficient to support SVZ neurogenesis, direct cell-cell contact between astrocytes and SVZ neuronal precursors may be necessary for the production of type A cells.
Proceedings of the National Academy of Sciences of the United States of America © 1999 National Academy of Sciences