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A Specific Complex between a Restriction Endonuclease and its DNA Substrate

Robert Yuan and Matthew Meselson
Proceedings of the National Academy of Sciences of the United States of America
Vol. 65, No. 2 (Feb. 15, 1970), pp. 357-362
Stable URL: http://www.jstor.org/stable/59647
Page Count: 6
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
A Specific Complex between a Restriction Endonuclease and its DNA Substrate
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Abstract

In the presence of Mg++, ATP, and S-adenosylmethionine, the DNA restriction endonuclease R· K forms a specific complex with its DNA substrate. The complex can be detected by its retention on nitrocellulose membranes.

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