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Mechanism of Activation of Catabolite-Sensitive Genes: A Positive Control System

Geoffrey Zubay, Daniele Schwartz and Jon Beckwith
Proceedings of the National Academy of Sciences of the United States of America
Vol. 66, No. 1 (May 15, 1970), pp. 104-110
Stable URL: http://www.jstor.org/stable/60191
Page Count: 7
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Mechanism of Activation of Catabolite-Sensitive Genes: A Positive Control System
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Abstract

Catabolite repression is defined as the inhibition of enzyme induction by glucose or related substances. In the bacterium E. coli, the effect of glucose appears to be due to a lowering of the cyclic AMP level. A DNA-directed cell-free system for β -galactosidase synthesis has served as a model system for studying the mechanism of action of cyclic AMP. Previously, it was reported that in this system cyclic AMP is required for normal initiation of mRNA synthesis. A protein factor which acts in conjunction with the cyclic AMP has been partially purified. This protein factor has a high affinity for cyclic AMP. These and other results presented herein lead us to the conclusion that cyclic AMP and a protein factor called the catabolite gene activator protein are part of a positive control system for activating catabolite-sensitive genes.

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