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Detection of Polyadenylic Acid Sequences in Viral and Eukaryotic RNA
Robert Sheldon, Christine Jurale and Joseph Kates
Proceedings of the National Academy of Sciences of the United States of America
Vol. 69, No. 2 (Feb., 1972), pp. 417-421
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/60937
Page Count: 5
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A rapid and specific technique to detect polyriboadenylic acid sequences in RNA is described. The method depends upon the ability of RNAs that contain poly(A) sequences to associate specifically with poly(U) that has been immobilized on fiberglass filters by ultraviolet irradiation. A high proportion of the transcripts synthesized in vivo and in vitro from the vaccinia virus genome contain poly(A) sequences and bind to the poly(U) filters. Similarly, DNA-like RNA from the nucleus and from the cytoplasmic polyribosomes of HeLa cells is rich in species that bind to poly(U) filters. Poly(U) immobilized on cellulose powder is useful to make columns with a high capacity for the binding and purification of poly(A)-containing RNAs.
Proceedings of the National Academy of Sciences of the United States of America © 1972 National Academy of Sciences