Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Induction of Auxotrophic Mutations by Treatment of Chinese Hamster Cells with 5-Bromodeoxyuridine and Black Light

E. H. Y. Chu, N. C. Sun and C. C. Chang
Proceedings of the National Academy of Sciences of the United States of America
Vol. 69, No. 11 (Nov., 1972), pp. 3459-3463
Stable URL: http://www.jstor.org/stable/61625
Page Count: 5
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Induction of Auxotrophic Mutations by Treatment of Chinese Hamster Cells with 5-Bromodeoxyuridine and Black Light
Preview not available

Abstract

Treatment of an aneuploid Chinese hamster cell line (V79) in tissue culture with 5-bromodeoxyuridine and black light, with or without prior exposure of cells to another mutagen, led to the isolation of auxotrophic mutants, each of which exhibits a specific nutritional requirement for glycine, uridine, purine, or for a combination of glycine, hypoxanthine, and thymidine. 61 mutants that could not use exogenous galactose were also isolated. Thus, this treatment functions as an inductive, but not as a selective, procedure for mutations in mammalian cells.

Page Thumbnails

  • Thumbnail: Page 
3459
    3459
  • Thumbnail: Page 
3460
    3460
  • Thumbnail: Page 
3461
    3461
  • Thumbnail: Page 
3462
    3462
  • Thumbnail: Page 
3463
    3463