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Induction of Auxotrophic Mutations by Treatment of Chinese Hamster Cells with 5-Bromodeoxyuridine and Black Light
E. H. Y. Chu, N. C. Sun and C. C. Chang
Proceedings of the National Academy of Sciences of the United States of America
Vol. 69, No. 11 (Nov., 1972), pp. 3459-3463
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/61625
Page Count: 5
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Treatment of an aneuploid Chinese hamster cell line (V79) in tissue culture with 5-bromodeoxyuridine and black light, with or without prior exposure of cells to another mutagen, led to the isolation of auxotrophic mutants, each of which exhibits a specific nutritional requirement for glycine, uridine, purine, or for a combination of glycine, hypoxanthine, and thymidine. 61 mutants that could not use exogenous galactose were also isolated. Thus, this treatment functions as an inductive, but not as a selective, procedure for mutations in mammalian cells.
Proceedings of the National Academy of Sciences of the United States of America © 1972 National Academy of Sciences