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The Use of Iodinated RNA for Gene Localization
Wolf Prensky, D. M. Steffensen and W. L. Hughes
Proceedings of the National Academy of Sciences of the United States of America
Vol. 70, No. 6 (Jun., 1973), pp. 1860-1864
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/62236
Page Count: 5
You can always find the topics here!Topics: RNA, Drosophila, Radioactive decay, Phosphates, Genetic hybridization, DNA, Chromosomes, Iodine, pH, Product labeling
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Carrier-free iodination of 5S RNA with 125I yields a probe suitable for use in cytological RNA-DNA hybridization studies. The genes coding for 5S ribosomal RNA in Drosophila could be localized by autoradiography after a 2-day exposure, whereas a 2-month exposure is needed when the best available [3H]RNA is used. The procedure introduces one covalently bound 125I atom per 100 nucleotides, resulting in a specific activity of over 108 dpm/μ g of RNA. The method may be readily applied to other problems involving molecular hybridization techniques.
Proceedings of the National Academy of Sciences of the United States of America © 1973 National Academy of Sciences