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Induction of Cytolysis of Cultured Lymphoma Cells by Adenosine 3′:5′-Cyclic Monophosphate and the Isolation of Resistant Variants
Violet Daniel, Gerald Litwack and Gordon M. Tomkins
Proceedings of the National Academy of Sciences of the United States of America
Vol. 70, No. 1 (Jan., 1973), pp. 76-79
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/62270
Page Count: 4
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Cultured mouse lymphosarcoma cells are killed on exposure to 0.1 mM N6,O2′-dibutyryl-adenosine 3′:5′-cyclic monophosphate. A population of cells resistant to the killing effect of dibutyryl cyclic AMP at concentrations as high as 1 mM was selected. The growth characteristics of the resistant cells were similar to those of the sensitive parental line. However, the resistant cells contain less cytoplasmic cyclic AMP-binding proteins and decreased cyclic AMP-stimulated protein kinase activity. It is proposed that transition from sensitivity to resistance to dibutyryl cyclic AMP in lymphoma cells is connected with a modification of the cyclic AMP-binding protein, which appears to be the regulatory subunit of the cyclic AMP-activated protein kinase.
Proceedings of the National Academy of Sciences of the United States of America © 1973 National Academy of Sciences