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RNA Polymerase from Bacillus amyloliquefaciens Infected with φ 29 Bacteriophage

Michael Holland and H. R. Whiteley
Proceedings of the National Academy of Sciences of the United States of America
Vol. 70, No. 8 (Aug., 1973), pp. 2234-2237
Stable URL: http://www.jstor.org/stable/62980
Page Count: 4
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
RNA Polymerase from Bacillus amyloliquefaciens Infected with φ 29 Bacteriophage
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Abstract

DNA-dependent RNA polymerase was purified from uninfected and φ 29-infected Bacillus amyloliquefaciens. Differences were observed in the specific activities, template specificities, stability, and sedimentation properties of the two enzymes. A polypeptide of 30,000 molecular weight was found in association with the polymerase of high specific activity from phage-infected cells and was absent from polymerase isolated from uninfected cells. The change in polymerase properties and the appearance of the polypeptide occurred early in phage infection.

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