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Pyridoxal Compounds as Specific Reagents for the α and β N-Termini of Hemoglobin
Ruth E. Benesch, Suzanna Yung, Tomokazu Suzuki, Christian Bauer and Reinhold Benesch
Proceedings of the National Academy of Sciences of the United States of America
Vol. 70, No. 9 (Sep., 1973), pp. 2595-2599
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/63060
Page Count: 5
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Bifunctional pyridoxal derivatives with a charged side chain in the 5′ position react specifically with the N-terminal valine residues of hemoglobin. Three of these compounds, which have only a single negative charge in the side chain, are highly selective for the α -chain N-terminal residues in the oxy conformation while pyridoxal phosphate, with two negative charges, reacts specifically with the β N-termini in deoxyhemoglobin. Schiff's-base formation, therefore, results in decreased oxygen affinity with pyridoxal phosphate but increased oxygen affinity with the other three compounds. Reduction with sodium borohydride leads to irreversible coupling at either end of the molecule, and preparation and properties of such derivatives are described.
Proceedings of the National Academy of Sciences of the United States of America © 1973 National Academy of Sciences