Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Pyridoxal Compounds as Specific Reagents for the α and β N-Termini of Hemoglobin

Ruth E. Benesch, Suzanna Yung, Tomokazu Suzuki, Christian Bauer and Reinhold Benesch
Proceedings of the National Academy of Sciences of the United States of America
Vol. 70, No. 9 (Sep., 1973), pp. 2595-2599
Stable URL: http://www.jstor.org/stable/63060
Page Count: 5
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Pyridoxal Compounds as Specific Reagents for the α  and β  N-Termini of Hemoglobin
Preview not available

Abstract

Bifunctional pyridoxal derivatives with a charged side chain in the 5′ position react specifically with the N-terminal valine residues of hemoglobin. Three of these compounds, which have only a single negative charge in the side chain, are highly selective for the α -chain N-terminal residues in the oxy conformation while pyridoxal phosphate, with two negative charges, reacts specifically with the β N-termini in deoxyhemoglobin. Schiff's-base formation, therefore, results in decreased oxygen affinity with pyridoxal phosphate but increased oxygen affinity with the other three compounds. Reduction with sodium borohydride leads to irreversible coupling at either end of the molecule, and preparation and properties of such derivatives are described.

Page Thumbnails

  • Thumbnail: Page 
2595
    2595
  • Thumbnail: Page 
2596
    2596
  • Thumbnail: Page 
2597
    2597
  • Thumbnail: Page 
2598
    2598
  • Thumbnail: Page 
2599
    2599