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Replication of the Escherichia coli Chromosome with a Soluble Enzyme System
Thomas Kornberg, Arthur Lockwood and A. Worcel
Proceedings of the National Academy of Sciences of the United States of America
Vol. 71, No. 8 (Aug., 1974), pp. 3189-3193
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/63218
Page Count: 5
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Semi-conservative DNA synthesis is observed when the isolated, folded E. coli chromosome is supplemented with a DNA-free, soluble enzyme fraction, the four deoxynucleoside 5′-triphosphates, ATP, and Mg++. The DNA synthesized in vitro remains associated with the folded chromosome during sedimentation through neutral sucrose, but is released as small DNA fragments in alkali. Sealing of these replicative intermediates to the chromosome requires the presence of both E. coli DNA polymerase I (EC 220.127.116.11) and DNA ligase (EC 18.104.22.168).
Proceedings of the National Academy of Sciences of the United States of America © 1974 National Academy of Sciences