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Cytoplasmic Inheritance of Chloramphenicol Resistance in Mouse Tissue Culture Cells
C. L. Bunn, Douglas C. Wallace and J. M. Eisenstadt
Proceedings of the National Academy of Sciences of the United States of America
Vol. 71, No. 5 (May, 1974), pp. 1681-1685
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/63501
Page Count: 5
You can always find the topics here!Topics: Cells, HeLa cells, Cell lines, Flasks, Chromosomes, Cell fusion, Mitochondrial DNA, Cytoplasmic inheritance, Hybridity, Cell nucleus
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A chloramphenicol-resistant mutant, isolated from mouse A9 cells, was enucleated and fused with a nucleated chloramphenicol-sensitive mouse cell line. Resultant fusion products, cytoplasmic hybrids (or ``cybrids''), were selected as resistant to chloramphenicol, and had the nuclear markers and chromosome complement of the chloramphenicol-sensitive parent. These cybrids appeared at the high frequency of 2-8 per 104 cells plated. Neither parent produced any colonies when plated under identical selective conditions. Fusion between enucleated chloramphenicol-sensitive cell fragments and the chloramphenicol-sensitive cell produced no resistant colonies, suggesting that chloramphenicol resistance is not due to an increase in the ratio of cytoplasm to nucleus. Furthermore, fusions between resistant and sensitive nucleated cells produced resistant hybrids at a frequency 100 times less than that of resistant cybrids. Thus, these stable chloramphenicol-resistant cybrids result from the fusion of a chloramphenicol-resistant cytoplasm with a chloramphenicol-sensitive cell. It is proposed, therefore, that chloramphenicol resistance is a cytoplasmically inherited characteristic in this mouse cell line.
Proceedings of the National Academy of Sciences of the United States of America © 1974 National Academy of Sciences