Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Enzymatic Utilization of P1-Di-N-acetylchitobiosyl P2-Dolichyl Pyrophosphate and its Chemical Synthesis

Josiah F. Wedgwood, Christopher D. Warren, Roger W. Jeanloz and Jack L. Strominger
Proceedings of the National Academy of Sciences of the United States of America
Vol. 71, No. 12 (Dec., 1974), pp. 5022-5026
Stable URL: http://www.jstor.org/stable/63962
Page Count: 5
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Enzymatic Utilization of P1-Di-N-acetylchitobiosyl P2-Dolichyl Pyrophosphate and its Chemical Synthesis
Preview not available

Abstract

Fully acetylated chitobiose was treated with phosphoric acid to give a mixture of products from which 2-acetamido-4-O-(2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-β -D-glucopyranosyl)-3,6-di-O-acetyl-2-deoxy - α ,β -D-glucopyranosyl phosphate (Ac3GlcNAc-Ac2GlcNAcP) was isolated by preparative thin layer chromatography. Treatment of this compound with P1-diphenyl P2-dolichyl pyrophosphate gave an acetylated pyrophosphate diester, which was purified chromatographically and deacetylated. The product, P1-di-N-acetylchitobiosyl P2-dolichyl pyrophosphate (Dol-P-P-GlcNAc-GlcNAc), was readily separated from P1-2-acetamido-2-deoxy-α -D-glucopyranosyl P2-dolichyl pyrophosphate (Dol-P-P-GlcNAc) by thin layer chromatography in several solvent systems. Addition of this compound to human lymphocyte membrane preparations led to the formation of a mannose-containing derivative which appears to be an oligosaccharide phospholipid, as judged by its behavior on DEAE-cellulose chromatography and by its hydrolysis to give an oligosaccharide containing more than four monosaccharide units.

Page Thumbnails

  • Thumbnail: Page 
5022
    5022
  • Thumbnail: Page 
5023
    5023
  • Thumbnail: Page 
5024
    5024
  • Thumbnail: Page 
5025
    5025
  • Thumbnail: Page 
5026
    5026