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New Chromosomal Location for Structural Genes of Ribosomal Proteins
Robert J. Watson, Jack Parker, Niels P. Fiil, Joel G. Flaks and James D. Friesen
Proceedings of the National Academy of Sciences of the United States of America
Vol. 72, No. 7 (Jul., 1975), pp. 2765-2769
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/64803
Page Count: 5
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An Escherichia coli mutant, ts9, previously reported by Flaks et al. (Cold Spring Harbor Symp. Quant. Biol. 31, 623-631, 1966) to have an electrophoretically altered ribosomal protein, has been further characterized and the altered component has been identified as L7/L12. Although mutant ts9 is temperature sensitive for growth (rts-), the rts and L7/L12 mutations are genetically separable and are both located between argH and rif. The L7/L12 mutation (rpyL) maps very close to relC, mutants of which have a defect in the 50S ribosomal subunit. The gene order is argH-rts-(rpyL, relC)-rif. Protein synthesis directed by bacteriophage λ cI857S7drifd18 in ultraviolet-irradiated cells indicates that L7/L12, as well as L1, L10, L11, and possibly L8 or L9 are coded by the phage DNA. Our results indicate that rpyL is the structural gene for L7/L12 and that this region of the E. coli chromosome contains a cluster of structural genes for ribosomal proteins.
Proceedings of the National Academy of Sciences of the United States of America © 1975 National Academy of Sciences