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Interaction of Lymphocytes with Lipid Bilayer Membranes: A Model for Lymphocyte-Mediated Lysis of Target Cells
Pierre Henkart and Robert Blumenthal
Proceedings of the National Academy of Sciences of the United States of America
Vol. 72, No. 7 (Jul., 1975), pp. 2789-2793
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/64808
Page Count: 5
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Horizontal lipid bilayer membranes were used as a model system to study lymphocyte-mediated killing of target cells. Dinitrophenylated lipid bilayers can physically support dozens of lymphocytes for periods of over one hour without breakage or increasing the electrical conductance of the membrane. However, in the presence of antibody against Dnp, human lymphocytes rapidly induced increases in membrane conductance of several orders of magnitude without membrane breakage. Such ionic permeability increases occurred only when the membrane voltage was positive on the lymphocyte side, as would be the case with a target cell membrane. The lymphocyte and antibody dependence of this conductance increase parallels that observed for lymphocyte killing of antibody-coated target cells. The results are interpreted as evidence that the primary event in lymphocyte killing of antibody-coated target cells is the creation of ion-conducting channels in the target membrane.
Proceedings of the National Academy of Sciences of the United States of America © 1975 National Academy of Sciences