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Transformation of Cultured Human Embryonic Fibroblasts by Oncornavirus-Like Particles Released from a Human Carcinoma Cell Line
Henny Balabanova, Moshe Kotler and Yechiel Becker
Proceedings of the National Academy of Sciences of the United States of America
Vol. 72, No. 7 (Jul., 1975), pp. 2794-2798
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/64809
Page Count: 5
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A fibroblast-like cell culture was established from a stomach biopsy of a patient with metastatic adenocarcinoma. One of the cultures, at the 6th passage level, left unattended for a month at 37 degrees, produced numerous foci of epithelioid cells. Upon subculturing, an epithelioid cell line, designated HCCL (human carcinoma cell line), was established. The HCCL cells released particles possessing the characteristics of oncornaviruses: density 1.175 g/ml, cores with a density of 1.22-1.26 g/ml, high-molecular-weight RNA (60-70S) and RNA-instructed DNA polymerase activity (deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase, EC 220.127.116.11). Inoculation of particles released from HCCL cells into cultures of human embryo muscle fibroblasts resulted in the appearance of foci of transformed cells.
Proceedings of the National Academy of Sciences of the United States of America © 1975 National Academy of Sciences