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In vitro Construction of Bacteriophage λ Carrying Segments of the Escherichia coli Chromosome: Selection of Hybrids Containing the Gene for DNA Ligase

John R. Cameron, Sharon M. Panasenko, I. R. Lehman and Ronald W. Davis
Proceedings of the National Academy of Sciences of the United States of America
Vol. 72, No. 9 (Sep., 1975), pp. 3416-3420
Stable URL: http://www.jstor.org/stable/65410
Page Count: 5
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
In vitro Construction of Bacteriophage λ  Carrying Segments of the Escherichia coli Chromosome: Selection of Hybrids Containing the Gene for DNA Ligase
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Abstract

DNA from λ gt-λ B bacteriophage was cleaved with EcoRI endonuclease and fragments from EcoRI-digested E. coli DNA were inserted. This DNA was used to infect E. coli, and phages containing the gene for DNA ligase were isolated by genetic selection. Two different hybrids were found with the same E. coli segment inserted in opposite orientations. Both hybrids produced similar levels of ligase as measured in crude extracts of infected cells.

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