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Hormone and Neurotransmitter Receptors in an Established Vascular Endothelial Cell Line
Vincenzo Buonassisi and J. Craig Venter
Proceedings of the National Academy of Sciences of the United States of America
Vol. 73, No. 5 (May, 1976), pp. 1612-1616
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/65961
Page Count: 5
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A cell line from the intima of the rabbit aorta has been established. This cell line exhibits strict contact inhibition, and morphologically resembles intimal endothelial cells. B-type blood group antigens and the presence of fibrinolytic activity also distinguish these cells from smooth muscle cells and from fibroblasts of the aortic wall. Endothelial cells were assayed for changes in levels of adenosine 3′:5′-cyclic monophosphate (cAMP) and guanosine 3′:5′-cyclic monophosphate (cGMP) in response to a series of vasoactive drugs. Control levels for cAMP and cGMP are 7.01 ± 0.82 and 1.50 ± 0.06, respectively (mean ± SEM). Norepinephrine, acetylcholine, 5-hydroxytryptamine, and phenylephrine increased the levels of both nucleotides significantly. Propranolol (10-5 M) and phentolamine (10-5 M) inhibited, respectively, the cAMP and cGMP response to norepinephrine. Angiotensin II and histamine significantly increased cGMP levels but not cAMP levels of the endothelial cells. The cGMP increases with acetylcholine were inhibited by atropine. These results indicate that the established cell line is endothelial in nature and contains cellular receptors to a variety of vasoactive agents.
Proceedings of the National Academy of Sciences of the United States of America © 1976 National Academy of Sciences