Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Hormone and Neurotransmitter Receptors in an Established Vascular Endothelial Cell Line

Vincenzo Buonassisi and J. Craig Venter
Proceedings of the National Academy of Sciences of the United States of America
Vol. 73, No. 5 (May, 1976), pp. 1612-1616
Stable URL: http://www.jstor.org/stable/65961
Page Count: 5
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Hormone and Neurotransmitter Receptors in an Established Vascular Endothelial Cell Line
Preview not available

Abstract

A cell line from the intima of the rabbit aorta has been established. This cell line exhibits strict contact inhibition, and morphologically resembles intimal endothelial cells. B-type blood group antigens and the presence of fibrinolytic activity also distinguish these cells from smooth muscle cells and from fibroblasts of the aortic wall. Endothelial cells were assayed for changes in levels of adenosine 3′:5′-cyclic monophosphate (cAMP) and guanosine 3′:5′-cyclic monophosphate (cGMP) in response to a series of vasoactive drugs. Control levels for cAMP and cGMP are 7.01 ± 0.82 and 1.50 ± 0.06, respectively (mean ± SEM). Norepinephrine, acetylcholine, 5-hydroxytryptamine, and phenylephrine increased the levels of both nucleotides significantly. Propranolol (10-5 M) and phentolamine (10-5 M) inhibited, respectively, the cAMP and cGMP response to norepinephrine. Angiotensin II and histamine significantly increased cGMP levels but not cAMP levels of the endothelial cells. The cGMP increases with acetylcholine were inhibited by atropine. These results indicate that the established cell line is endothelial in nature and contains cellular receptors to a variety of vasoactive agents.

Page Thumbnails

  • Thumbnail: Page 
1612
    1612
  • Thumbnail: Page 
1613
    1613
  • Thumbnail: Page 
1614
    1614
  • Thumbnail: Page 
1615
    1615
  • Thumbnail: Page 
1616
    1616