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Induction of Sigma Factor Synthesis in Escherichia coli by the N Gene Product of Bacteriophage Lambda
Yoshikazu Nakamura and Takashi Yura
Proceedings of the National Academy of Sciences of the United States of America
Vol. 73, No. 12 (Dec., 1976), pp. 4405-4409
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/66121
Page Count: 5
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Thermoinduction of cells of E. coli carrying prophage λ cI857 within the bfe gene brings about not only ``escape synthesis'' of core subunits of the DNA-dependent RNA polymerase (RNA nucleotidyltransferase, nucleosidetriphosphate:RNA nucleotidyltransferase, EC 22.214.171.124), but also a striking stimulation of σ factor synthesis. The latter phenomenon, termed σ induction, is generally observed after λ phage infection or prophage induction. A series of experiments with various bacterial and phage strains led us to conclude that the N gene product of λ is directly involved in the σ induction. These and other results obtained with mutants defective in transcription termination factor ρ suggest the involvement of a ρ -sensitive site in the control of σ gene expression in E. coli.
Proceedings of the National Academy of Sciences of the United States of America © 1976 National Academy of Sciences