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Membrane-Associated Assembly of M13 Phage in Extracts of Virus-Infected Escherichia coli
William Wickner and Teresa Killick
Proceedings of the National Academy of Sciences of the United States of America
Vol. 74, No. 2 (Feb., 1977), pp. 505-509
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/66218
Page Count: 5
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Assembly of coliphage M13 is known to occur as the viral DNA crosses the cytoplasmic membrane, shedding its virus-coded DNA unwinding protein and acquiring from the membrane approximately 2400 copies of the major coat protein. Conditions are described in which extracts of M13-infected E. coli and membranes prepared from such extracts will support virus assembly at a rate equivalent to that of intact cells. Extracts prepared from cells infected with temperature-sensitive M13 mutants in genes 1, 3, 4, or 5 are temperature-sensitive in this cell-free assembly reaction. Phage assembly in vitro requires magnesium and as yet an unidentified heat-stable cofactor of low molecular weight. The rate of virus assembly is approximately linear with respect to extract concentration over a 104-fold range, consistent with the observation that the entire M13 assembly activity copurifies with the cell membrane fraction.
Proceedings of the National Academy of Sciences of the United States of America © 1977 National Academy of Sciences