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Membrane-Associated Assembly of M13 Phage in Extracts of Virus-Infected Escherichia coli

William Wickner and Teresa Killick
Proceedings of the National Academy of Sciences of the United States of America
Vol. 74, No. 2 (Feb., 1977), pp. 505-509
Stable URL: http://www.jstor.org/stable/66218
Page Count: 5
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Membrane-Associated Assembly of M13 Phage in Extracts of Virus-Infected Escherichia coli
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Abstract

Assembly of coliphage M13 is known to occur as the viral DNA crosses the cytoplasmic membrane, shedding its virus-coded DNA unwinding protein and acquiring from the membrane approximately 2400 copies of the major coat protein. Conditions are described in which extracts of M13-infected E. coli and membranes prepared from such extracts will support virus assembly at a rate equivalent to that of intact cells. Extracts prepared from cells infected with temperature-sensitive M13 mutants in genes 1, 3, 4, or 5 are temperature-sensitive in this cell-free assembly reaction. Phage assembly in vitro requires magnesium and as yet an unidentified heat-stable cofactor of low molecular weight. The rate of virus assembly is approximately linear with respect to extract concentration over a 104-fold range, consistent with the observation that the entire M13 assembly activity copurifies with the cell membrane fraction.

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