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An Approach to the Targeted Attachment of Peptides and Proteins to Solid Supports
Klaus Hofmann and Yoshiaki Kiso
Proceedings of the National Academy of Sciences of the United States of America
Vol. 73, No. 10 (Oct., 1976), pp. 3516-3518
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/66631
Page Count: 3
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A novel approach to affinity columns is described that is based on the high avidity of biotinylated molecules for avidin attached to solid supports. Biocytin amide [Nε-(+)-biotinyllysine amide] was coupled to the COOH-terminal carboxyl group of corticotropin(1-24) [ACTH(1-24)] to form [biocytin25]ACTH(1-25) amide. The ability of this peptide to stimulate steroidogenesis of bovine adrenocortical cells was within experimental error identical to that of ACTH(1-24). The peptide also binds to avidin and avidin-Sepharose, forming stable complexes. Thus, with biotin as the anchor, the adrenocorticotropically active segment of the ACTH molecule was attached to a solid support in a targeted manner. The general applicability of this principle for the attachment of peptides and proteins to solid supports is discussed.
Proceedings of the National Academy of Sciences of the United States of America © 1976 National Academy of Sciences