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5′-terminal Sequences and Coding Region of Late Simian Virus 40 mRNAs are Derived from Noncontiguous Segments of the Viral Genome
Sara Lavi and Yoram Groner
Proceedings of the National Academy of Sciences of the United States of America
Vol. 74, No. 12 (Dec., 1977), pp. 5323-5327
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/67346
Page Count: 5
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The region of the simian virus 40 genome complementary to the 5′ end of the most abundant poly(A)-containing 19S and 16S mRNAs was mapped by hybridization of double-labeled RNA ([3H]methyl group and [14C]uridine) to specific DNA fragments. Chemical identification of methylated residues indicated that a common ``leader'' sequence adjacent to the 5′ terminus of both 19S and 16S mRNA is transcribed from DNA sequences located between 0.67 and 0.76 map units. The estimated size of this ``leader'' RNA, which does not code for any known viral protein, is 170-200 nucleotides. Our results indicate that sequences complementary to the ``leader'' region and coding portion of 16S mRNA are located in separate parts of the simian virus 40 genome.
Proceedings of the National Academy of Sciences of the United States of America © 1977 National Academy of Sciences