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Rescue of Immunoglobulin Secretion from Human Neoplastic Lymphoid Cells by Somatic Cell Hybridization
Ronald Levy and Jeanette Dilley
Proceedings of the National Academy of Sciences of the United States of America
Vol. 75, No. 5 (May, 1978), pp. 2411-2415
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/68456
Page Count: 5
You can always find the topics here!Topics: Immunoglobulins, Lymphocytes, Somatic cells, Molecules, B lymphocytes, Molecular chains, Cell lines, Gels, Secretion, Materials
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B leukemia cells from four different patients were hybridized with a mouse myeloma cell line with polyethylene glycol as a fusing agent. The original leukemia cells all expressed immunoglobulin on their surface, but failed to secrete it. Over 200 different human-mouse somatic cell hybrids were obtained; 57% of them secreted human immunoglobulin in large amounts. Human immunoglobulin secretion can be a stable property of these hybrid cells over months of continuous culture. In each case the human immunoglobulin secreted was restricted to the light chain type expressed by the parental B leukemia cell. In addition, these hybrid cells secreted the original mouse myeloma protein and a variety of mixed human-mouse immunoglobulin molecules.
Proceedings of the National Academy of Sciences of the United States of America © 1978 National Academy of Sciences