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Temperature-Sensitive Replication of H5ts125 Adenovirus DNA in vitro

Marshall S. Horwitz
Proceedings of the National Academy of Sciences of the United States of America
Vol. 75, No. 9 (Sep., 1978), pp. 4291-4295
Stable URL: http://www.jstor.org/stable/68535
Page Count: 5
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Temperature-Sensitive Replication of H5ts125 Adenovirus DNA in vitro
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Abstract

A soluble adenovirus DNA replication system has been prepared in extracts of HeLa cells infected with the temperature-sensitive mutant H5ts125. These nuclear extracts synthesize full-sized viral DNA at 30 degrees but are temperature sensitive in this function at 38 degrees. A 72,000-dalton DNA-binding protein purified from cells infected with wild-type virus complements the temperature-sensitive defect and restores viral DNA replication to 74% of normal values.

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