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Sodium Pyrophosphate Inhibition of RNA· DNA Hybrid Degradation by Reverse Transcriptase

Jeanne C. Myers and S. Spiegelman
Proceedings of the National Academy of Sciences of the United States of America
Vol. 75, No. 11 (Nov., 1978), pp. 5329-5333
Stable URL: http://www.jstor.org/stable/68623
Page Count: 5
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Sodium Pyrophosphate Inhibition of RNA· DNA Hybrid Degradation by Reverse Transcriptase
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Abstract

Sodium pyrophosphate inhibits synthesis of anticomplementary DNA during a reverse transcriptase (RNA-directed DNA nucleotidyltransferase, EC 2.7.7.7) catalyzed reaction. In the presence of pyrophosphate, the complementary DNA remains stably complexed to the RNA template. In the absence of pyrophosphate, the DNA· RNA hybrid template is degraded and anticomplementary DNA is synthesized. High concentrations of additives containing phosphodiester bonds appear to inhibit the ribonuclease H activity (hybrid nuclease, EC 3.1.4.34) of the reverse transcriptase, thereby preventing formation of RNA primers necessary for the synthesis of anticomplementary DNA.

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