You are not currently logged in.
Access JSTOR through your library or other institution:
If You Use a Screen ReaderThis content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Sodium Pyrophosphate Inhibition of RNA· DNA Hybrid Degradation by Reverse Transcriptase
Jeanne C. Myers and S. Spiegelman
Proceedings of the National Academy of Sciences of the United States of America
Vol. 75, No. 11 (Nov., 1978), pp. 5329-5333
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/68623
Page Count: 5
You can always find the topics here!Topics: Hybridity, DNA, Polio, RNA, Sodium, Complementary DNA, Diphosphates, Sulfates, Cesium, Legends
Were these topics helpful?See something inaccurate? Let us know!
Select the topics that are inaccurate.
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Preview not available
Sodium pyrophosphate inhibits synthesis of anticomplementary DNA during a reverse transcriptase (RNA-directed DNA nucleotidyltransferase, EC 220.127.116.11) catalyzed reaction. In the presence of pyrophosphate, the complementary DNA remains stably complexed to the RNA template. In the absence of pyrophosphate, the DNA· RNA hybrid template is degraded and anticomplementary DNA is synthesized. High concentrations of additives containing phosphodiester bonds appear to inhibit the ribonuclease H activity (hybrid nuclease, EC 18.104.22.168) of the reverse transcriptase, thereby preventing formation of RNA primers necessary for the synthesis of anticomplementary DNA.
Proceedings of the National Academy of Sciences of the United States of America © 1978 National Academy of Sciences