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Lengthening of the G1 Phase is not Strictly Correlated with Differentiation in Friend Erythroleukemia Cells
Eileen A. Friedman and Carl L. Schildkraut
Proceedings of the National Academy of Sciences of the United States of America
Vol. 75, No. 8 (Aug., 1978), pp. 3813-3817
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/68773
Page Count: 5
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Friend murine erythroleukemia cells (Friend cells) undergo erythroid differentiation in vitro with an increased probability when cells are cultured in the presence of dimethyl sulfoxide (Me2SO) or other agents. Exponentially growing Friend cells, after dilution into medium containing Me2SO, underwent a transient lengthening of the G1 phase of the cell cycle before they became committed to erythroid differentiation. For nine inducing agents, a positive correlation was found between the percentage of cells that had differentiated and synthesized heme, and the percentage of progenitor cells in which a lengthened G1 phase had previously been observed. This correlation was not found, however, with two other potent inducing agents, hypoxanthine and actinomycin D. Moreover, cells that underwent a lengthened G1 phase did not always terminally differentiate. One such example was a Me2SO-resistant, variant Friend cell line (520a) grown in the presence of Me2SO. These results imply that the prolonged G1 phase, although observed with many inducers, is not a prerequisite for erythroid differentiation with all inducers.
Proceedings of the National Academy of Sciences of the United States of America © 1978 National Academy of Sciences