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Demonstration of an Ecdysteroid Receptor in a Cultured Cell Line of Drosophila melanogaster

Peter Maroy, Roger Dennis, Christoph Beckers, Becky A. Sage and John D. O'Connor
Proceedings of the National Academy of Sciences of the United States of America
Vol. 75, No. 12 (Dec., 1978), pp. 6035-6038
Stable URL: http://www.jstor.org/stable/68894
Page Count: 4
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Demonstration of an Ecdysteroid Receptor in a Cultured Cell Line of Drosophila melanogaster
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Abstract

Incubation of the high-speed supernatant from the Kc cell line of Drosophila melanogaster with [3H]ponasterone A results in significant binding of the ligand as determined by gel filtration and dextran-coated charcoal binding assays. In vivo exposure of the Kc line to [3H]ponasterone A for 30 min results in a marked binding of the ligand by a KCl-soluble nuclear extract. With both the cytosol and nuclear preparations the binding has specificity and a low dissociation constant (3 × 10-9 M). In addition the labeling of the nuclear preparation exhibits a saturation at approximately 7 × 10-10 M which probably reflects the molar concentration of cytoplasmic receptors.

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