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Demonstration of an Ecdysteroid Receptor in a Cultured Cell Line of Drosophila melanogaster
Peter Maroy, Roger Dennis, Christoph Beckers, Becky A. Sage and John D. O'Connor
Proceedings of the National Academy of Sciences of the United States of America
Vol. 75, No. 12 (Dec., 1978), pp. 6035-6038
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/68894
Page Count: 4
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Incubation of the high-speed supernatant from the Kc cell line of Drosophila melanogaster with [3H]ponasterone A results in significant binding of the ligand as determined by gel filtration and dextran-coated charcoal binding assays. In vivo exposure of the Kc line to [3H]ponasterone A for 30 min results in a marked binding of the ligand by a KCl-soluble nuclear extract. With both the cytosol and nuclear preparations the binding has specificity and a low dissociation constant (3 × 10-9 M). In addition the labeling of the nuclear preparation exhibits a saturation at approximately 7 × 10-10 M which probably reflects the molar concentration of cytoplasmic receptors.
Proceedings of the National Academy of Sciences of the United States of America © 1978 National Academy of Sciences