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Sleep-Promoting Factor S: Purification and Properties
J. M. Krueger, J. R. Pappenheimer and M. L. Karnovsky
Proceedings of the National Academy of Sciences of the United States of America
Vol. 75, No. 10 (Oct., 1978), pp. 5235-5238
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/69097
Page Count: 4
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Sleep-promoting factor was purified from acid/acetone extracts of whole brains of rabbits and from brainstems of slaughterhouse cattle. Intraventricular infusion of extracts purified by means of ion exchange and gel filtration induced excess slow-wave sleep in rabbits for 5-10 hr. The procedure is simple and provides material suitable for physiological studies. Further treatment by partition chromatography and electrophoresis yielded an active product that was purified at least 1 million-fold. This product was inactivated by incubation with mixed carboxypeptidases A and B. Amino acid analysis of acid hydrolysates indicated that the effective dose was less than 150 pmol per rabbit and the original concentration in brain tissue was of the order of 30 pmol/g of brain.
Proceedings of the National Academy of Sciences of the United States of America © 1978 National Academy of Sciences