Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

In vitro Transformation of Mouse Bone Marrow Cells by the Polycythemic Strain of Friend Leukemia Virus

R. Revoltella, L. Bertolini and C. Friend
Proceedings of the National Academy of Sciences of the United States of America
Vol. 76, No. 3 (Mar., 1979), pp. 1464-1468
Stable URL: http://www.jstor.org/stable/69307
Page Count: 5
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
In vitro Transformation of Mouse Bone Marrow Cells by the Polycythemic Strain of Friend Leukemia Virus
Preview not available

Abstract

Strains of Friend leukemia virus (FLV) that are associated with polycythemia contain the defective spleen focus-forming virus (SFFV). To determine whether the transforming ability of FLV was affected by the presence of this second agent, DBA/2J mouse bone marrow cells were infected in vitro. Criteria for transformation were the establishment of permanent lines, growth on semisolid agarose, and the production of tumors at the site of inoculation in syngeneic hosts. Two lines of immature hematopoietic cells that grow in suspension originated from the infected cultures. Each has an almost diploid karyotype (38-39 chromosomes) and 3-4 metacentric chromosomes. These transformed cells express gp71 viral envelope glycoprotein and p30 viral core protein antigens. Virus production was measured by reverse transcriptase (RNA-dependent DNA polymerase) activity of the virions released into the medium. The virus, assayed in vivo for infectivity, has SFFV activity but is attenuated for leukemogenicity. The stimulation of hemoglobin synthesis in the cells grown in medium supplemented with dimethyl sulfoxide or hexamethylene bisacetamide indicates that the cells are erythroid in origin. SFFV may have a function analogous to erythropoietin in influencing the process of transformation by FLV.

Page Thumbnails

  • Thumbnail: Page 
1464
    1464
  • Thumbnail: Page 
1465
    1465
  • Thumbnail: Page 
1466
    1466
  • Thumbnail: Page 
1467
    1467
  • Thumbnail: Page 
1468
    1468