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Migratory Patterns of Cloned Neural Crest Melanocytes Injected into Host Chicken Embryos
Marianne E. Bronner and Alan M. Cohen
Proceedings of the National Academy of Sciences of the United States of America
Vol. 76, No. 4 (Apr., 1979), pp. 1843-1847
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/69610
Page Count: 5
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Cloned quail melanocytes grown in tissue culture for 8 days or more were injected into 21/2-day-old chicken embryos. The pigment cells were placed directly into the somitic lumen by means of an injection micropipette. This technique for introducing marked neural crest cells into host embryos causes far less damage than previous methods which require extirpation and replacement of the neural tube. In addition, small numbers of homogeneous cells can be implanted by this procedure. When injected into one of the posterior somites, cultured pigment cells migrated along the ventral neural crest pathway. Three days after injection the melanocytes had migrated ventral to the dorsal root ganglia and prevertebral and primary sympathetic chain ganglia and were seen associated with the adrenal gland and aortic plexi. Melanocytes were frequently found in or adjacent to the gonads and often had migrated as far as the gut.
Proceedings of the National Academy of Sciences of the United States of America © 1979 National Academy of Sciences