Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Synthesis and Processing of Human Chorionic Gonadotropin Subunits in Cultured Choriocarcinoma Cells

Raymond W. Ruddon, Charlotte A. Hanson and Nancy J. Addison
Proceedings of the National Academy of Sciences of the United States of America
Vol. 76, No. 10 (Oct., 1979), pp. 5143-5147
Stable URL: http://www.jstor.org/stable/70404
Page Count: 5
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Synthesis and Processing of Human Chorionic Gonadotropin Subunits in Cultured Choriocarcinoma Cells
Preview not available

Abstract

Pulse and pulse-chase experiments have identified the presence of partially glycosylated precursors of the $\alpha $ and $\beta $ subunits of human chorionic gonadotropin (hCG) in cultured JAR choriocarcinoma cells. The $\alpha $ subunit precursor has an apparent molecular weight (by sodium dodecyl sulfate/polyacrylamide gel electrophoresis) of 18,000 (compared to 22,000 for fully processed $\alpha $ subunit); the $\beta $ subunit precursor has an apparent molecular weight of 24,000 (fully processed, 34,000). Both of these precursors appear to have an intracellular half-life of at least 1 hr and to contain the mannose core but not the terminal carbohydrate sequences. Fully processed $\alpha $ and $\beta $ subunits do not accumulate intracellularly, indicating that further processing of the precursors is followed by rapid secretion.

Page Thumbnails

  • Thumbnail: Page 
5143
    5143
  • Thumbnail: Page 
5144
    5144
  • Thumbnail: Page 
5145
    5145
  • Thumbnail: Page 
5146
    5146
  • Thumbnail: Page 
5147
    5147