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The Utilization of Glucose in the Brain and Other Organs of the Cat
M. K. Gaitonde, S. A. Marchi and D. Richter
Proceedings of the Royal Society of London. Series B, Biological Sciences
Vol. 160, No. 978 (Apr. 14, 1964), pp. 124-136
Published by: Royal Society
Stable URL: http://www.jstor.org/stable/75395
Page Count: 13
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After subcutaneous injection of [14C]glucose in the cat the total 14C content per gram fresh tissue was relatively high in liver, kidney, blood and brain: lower values were obtained in heart, spleen, lung, skeletal mucle and spinal cord. In all organs examined more than 95% of the radioactivity present at 22 min after injection was contained in the acid-soluble fraction of the tissue: proteins, lipids and nucleic acids together accounted for only 0· 2 to 5% of the radioactivity. In most organs the 14C in the acid-soluble fraction was present mainly as [14C]glucose, but in nervous tissues a large part (48 to 74%) of the 14C was contained in the free amino acid fraction. The incorporation of 14C from [14C]glucose into amino acids (counts min-1 g fresh tissue-1) in vivo was highest in the cerebral cortex and decreased in the order cerebral cortex > cerebellum > pons and medulla > spinal cord: the incorporation into amino acids was several times greater in the brain than in other organs examined. Values obtained for the heart were intermediate between those for brain and other organs. About 80% of the 14C incorporated into amino acids of the cerebral cortex was combined in glutamic and aspartic acids. In liver, spleen, muscle, lung and blood the basic and neutral amino acids accounted for a relatively larger proportion of the radioactivity of the amino acid fraction. The 14C contained in tricarboxylic acid cycle intermediates accounted for 20 to 32% of the radioactivity of the acid-soluble fraction in different parts of the brain.
Proceedings of the Royal Society of London. Series B, Biological Sciences © 1964 Royal Society