Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Formylmethionyl-tRNA Alters RNA Polymerase Specificity

Paul G. Debenham, Olaf Pongs and Andrew A. Travers
Proceedings of the National Academy of Sciences of the United States of America
Vol. 77, No. 2, [Part 2: Biological Sciences] (Feb., 1980), pp. 870-874
Stable URL: http://www.jstor.org/stable/8340
Page Count: 5
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Formylmethionyl-tRNA Alters RNA Polymerase Specificity
Preview not available

Abstract

Escherichia coli fMet-tRNAf Met alters the pattern of promoter selection of E. coli RNA polymerase (RNA nucleotidyltransferase, nucleosidetriphosphate:RNA nucleotidyltransferase; EC 2.7.7.6), affecting RNA synthesis from the rRNA, suIII + tRNA, and lac promoters in different ways. The in vitro synthesis of the stable RNA species is selectively decreased, whereas that of lac RNA from both the wild-type and mutant UV5 promoters is selectively increased at high ionic strength. The functional effect of fMet-tRNAf Met resembles that of the nucleotide guanosine 3′-diphosphate 5′-diphosphate (ppGpp). This nucleotide competes with the binding of fMet-tRNAf Met to RNA polymerase.

Page Thumbnails

  • Thumbnail: Page 
870
    870
  • Thumbnail: Page 
871
    871
  • Thumbnail: Page 
872
    872
  • Thumbnail: Page 
873
    873
  • Thumbnail: Page 
874
    874