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Culture of Hormone-Dependent Functional Epithelial Cells from Rat Thyroids

F. S. Ambesi-Impiombato, L. A. M. Parks and H. G. Coon
Proceedings of the National Academy of Sciences of the United States of America
Vol. 77, No. 6, [Part 2: Biological Sciences] (Jun., 1980), pp. 3455-3459
Stable URL: http://www.jstor.org/stable/8908
Page Count: 5
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Culture of Hormone-Dependent Functional Epithelial Cells from Rat Thyroids
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Abstract

Primary cultures of rat thyroid cells were made in medium supplemented with 0.1-0.5% calf serum and containing six hormones or growth factors: insulin, thyrotropin, transferrin, hydrocortisone, somatostatin, and glycyl-L-histidyl-L-lysine acetate. The FRTL strain was purified by successive colonial isolations and was found to maintain highly differentiated features (secretion into the culture medium of physiological amounts of thyroglobulin and concentration of iodide by 100-fold). The FRTL strain has been observed for more than 3 years in continuous culture. It has maintained the same biochemical and morphological characteristics that typified the primary cultures of thyroid follicular cells immediately after their enzymatic release from the rat thyroid. Thyroid epithelial cells that were grown under more conventional cell culture conditions failed to retain these specialized characteristics. We show that maintenance in vitro of these specialized functions of rat thyroid follicular cells is dependent on low serum concentrations and supplementation with hormones in the primary cultures. Our observations indicate that this culture strategem may be applicable to the general problem of maintenance of differentiated characteristics in cultures of other epithelial cells.

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