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Microtubule Assembly and Concanavalin A Capping in Lymphocytes: Reappraisal Using Normal and Abnormal Human Peripheral Blood Cells

Janet M. Oliver, Erwin W. Gelfand, Christine B. Pearson, Janet R. Pfeiffer and Hans-Michael Dosch
Proceedings of the National Academy of Sciences of the United States of America
Vol. 77, No. 6, [Part 2: Biological Sciences] (Jun., 1980), pp. 3499-3503
Stable URL: http://www.jstor.org/stable/8917
Page Count: 5
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Microtubule Assembly and Concanavalin A Capping in Lymphocytes: Reappraisal Using Normal and Abnormal Human Peripheral Blood Cells
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Abstract

We have analyzed the assembly of microtubules and the distribution of concanavalin A (Con A)-receptor complexes in the same populations of human peripheral blood T and B lymphocytes. We hoped to resolve the prolonged controversy over the relationship of microtubules to Con A cap formation in lymphocytes and to explain the abnormally high spontaneous and colchicine-induced Con A capping that was observed recently in lymphocytes from a patient with an inherited form of severe combined immunodeficiency disease (SCID) characterized by total immunologic dysfunction despite normal numbers and distribution of T and B cells. The data establish that (i) microtubule disassembly is correlated with enhanced Con A cap formation on normal human lymphocytes; (ii) T and B cells differ significantly from each other and from circulating polymorphonuclear leukocytes with respect to their capping responses after exposure to colchicine; and (iii) there is an abnormal relationship of microtubule assembly to surface topography in the functionally defective SCID cells.

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