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Microtubule Assembly and Concanavalin A Capping in Lymphocytes: Reappraisal Using Normal and Abnormal Human Peripheral Blood Cells
Janet M. Oliver, Erwin W. Gelfand, Christine B. Pearson, Janet R. Pfeiffer and Hans-Michael Dosch
Proceedings of the National Academy of Sciences of the United States of America
Vol. 77, No. 6, [Part 2: Biological Sciences] (Jun., 1980), pp. 3499-3503
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/8917
Page Count: 5
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We have analyzed the assembly of microtubules and the distribution of concanavalin A (Con A)-receptor complexes in the same populations of human peripheral blood T and B lymphocytes. We hoped to resolve the prolonged controversy over the relationship of microtubules to Con A cap formation in lymphocytes and to explain the abnormally high spontaneous and colchicine-induced Con A capping that was observed recently in lymphocytes from a patient with an inherited form of severe combined immunodeficiency disease (SCID) characterized by total immunologic dysfunction despite normal numbers and distribution of T and B cells. The data establish that (i) microtubule disassembly is correlated with enhanced Con A cap formation on normal human lymphocytes; (ii) T and B cells differ significantly from each other and from circulating polymorphonuclear leukocytes with respect to their capping responses after exposure to colchicine; and (iii) there is an abnormal relationship of microtubule assembly to surface topography in the functionally defective SCID cells.
Proceedings of the National Academy of Sciences of the United States of America © 1980 National Academy of Sciences