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Structural Organization of Human Genomic DNA Encoding the Pro-Opiomelanocortin Peptide
Annie C. Y. Chang, Madeleine Cochet and Stanley N. Cohen
Proceedings of the National Academy of Sciences of the United States of America
Vol. 77, No. 8, [Part 2: Biological Sciences] (Aug., 1980), pp. 4890-4894
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/9214
Page Count: 5
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We have isolated a human genomic DNA segment encoding the corticotropin-β -lipotropin precursor peptide from a fetal DNA library, using previously cloned bovine cDNA for this peptide as a probe. The human genomic DNA was studied by electron microscope heteroduplex analysis and gel blotting methods, and its nucleotide sequence was determined and compared with that of cDNA corresponding to bovine pro-opiomelanocortin mRNA. From this sequence, segments of interspecies conservation and divergence, punctuated by pairs of the basic amino acid residues lysine and arginine, were identified. No noncoding intervening sequence was observed over an 830-base-pair DNA segment extending from a position near the 5′ end of the structural pro-opiomelanocortin gene through the 3′ terminus of the cDNA and including sequences for the component peptide hormones corticotropin and β -lipotropin.
Proceedings of the National Academy of Sciences of the United States of America © 1980 National Academy of Sciences