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Identification of Two Escherichia coli Factor Y Effector Sites near the Origina of Replication of the Plasmids ColE1 and pBR322
S. L. Zipursky and K. J. Marians
Proceedings of the National Academy of Sciences of the United States of America
Vol. 77, No. 11, [Part 2: Biological Sciences] (Nov., 1980), pp. 6521-6525
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/9588
Page Count: 5
You can always find the topics here!Topics: DNA, Adenosine triphosphatases, Enzymes, Plasmids, Replication origin, RNA, Biochemistry, Gels, Nucleotides, Duplexes
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The Escherichia coli replication factor Y has been characterized as a φ X174 (+) strand specific DNA-dependent phosphohydrolase. In conjunction with other E. coli replication proteins, factor Y is involved in the formation of heterogeneous primers that are elongated by the E. coli DNA polymerase III elongation machinery. We report here that the heat-denatured DNAs of plasmids pBR322 and ColE1 serve as effectors for the hydrolysis of ATP by factor Y. The DNA sequences of pBR322 responsible for factor Y effector activity have been localized. Two separate regions of the pBR322 chromosome support Y ATPase activity. These sequences are near the replication origin and are located on opposite DNA strands.
Proceedings of the National Academy of Sciences of the United States of America © 1980 National Academy of Sciences