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Estrogenic and DNA-Damaging Activity of Red No. 3 in Human Breast Cancer Cells

Craig Dees, Minoo Askari, Scott Garrett, Kellie Gehrs, Don Henley and C. Murray Ardies
Environmental Health Perspectives
Vol. 105, Supplement 3: Breast Cancer (Apr., 1997), pp. 625-632
DOI: 10.2307/3433381
Stable URL: http://www.jstor.org/stable/3433381
Page Count: 8
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Estrogenic and DNA-Damaging Activity of Red No. 3 in Human Breast Cancer Cells
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Abstract

Exposure to pesticides, dyes, and pollutants that mimic the growth promoting effects of estrogen may cause breast cancer. The pesticide DDT and the food colorant Red No. 3 were found to increase the growth of HTB 133 but not estrogen receptor (ER) negative human breast cells (HTB 125) or rat liver epithelial cells (RLE). Red No. 3, β-estradiol, and DDT increase ER site-specific DNA binding to the estrogen response element in HTB 133 cells and increase cyclin-dependent kinase 2 activity in MCF-7 breast cancer cells. Site-specific DNA binding by p53 in RLE, HTB 125, HTB 133, and MCF-7 cells was increased when they were treated with Red No. 3, which suggests that cellular DNA was damaged by this colorant. Red No. 3 increased binding of the ER from MCF-7 cells to the estrogen-responsive element. Consumption of Red No. 3, which has estrogenlike growth stimulatory properties and may be genotoxic, could be a significant risk factor in human breast carcinogenesis.

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