With a personal account, you can read up to 100 articles each month for free.
Already have an account?
- Access everything in the JPASS collection
- Read the full-text of every article
- Download up to 10 article PDFs to save and keep
- Access everything in the JPASS collection
- Read the full-text of every article
- Download up to 120 article PDFs to save and keep
A sequence of 50 residues in f1 DNA has been determined by the extension of a chemically synthesized octadeoxyribonucleotide by Escherichia coli DNA polymerase I, with radioactive nucleoside triphosphates and f1 DNA template. The polymerized product was synthesized either in the presence of manganese and a mixture of ribo- and deoxyribotriphosphates or in a magnesium-containing reaction with one or more of the four triphosphates absent. The sequence determination depended largely on fractionation of the polymerized products by two-dimensional ``homochromatography.'' This approach and the techniques for the subsequent sequence analysis should be of general use for determining other sequences of DNA. Several features of this sequence suggest that it is located in an intercistronic region of f1 DNA.
PNAS is the world's most-cited multidisciplinary scientific serial. It publishes high-impact research reports, commentaries, perspectives, reviews, colloquium papers, and actions of the Academy. In accordance with the guiding principles established by George Ellery Hale in 1914, PNAS publishes brief first announcements of Academy Members' and Foreign Associates' more important contributions to research and of work that appears to a Member to be of particular importance.
The National Academy of Sciences (NAS) is a private, nonprofit organization of the country’s leading researchers. The NAS recognizes and promotes outstanding science through election to membership; publication in its journal, PNAS; and its awards, programs, and special activities. Through the National Academies of Sciences, Engineering, and Medicine, the NAS provides objective, science-based advice on critical issues affecting the nation.
This item is part of a JSTOR Collection.
For terms and use, please refer to our
Proceedings of the National Academy of Sciences of the United States of America
© 1973 National Academy of Sciences