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Chromosome Measurement and Sorting by Flow Systems
J. W. Gray, A. V. Carrano, L. L. Steinmetz, M. A. Van Dilla, D. H. Moore, B. H. Mayall and M. L. Mendelsohn
Proceedings of the National Academy of Sciences of the United States of America
Vol. 72, No. 4 (Apr., 1975), pp. 1231-1234
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/64823
Page Count: 4
You can always find the topics here!Topics: Chromosomes, Cell lines, Metaphase, Chromosome morphology, Cell membranes, Fluorescence, Bromides, Systems analysis, Cells, Karyotype
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A flow microfluorometer was used to measure metaphase chromosomes in suspension at rates up to 100,000 per min. Chromosomes from cells of the Chinese hamster M3-1 cell line were isolated, stained for DNA with the fluorescent dye ethidium bromide, and analyzed for DNA content. Nine distinct peaks were resolved that correspond well with independent chromosomal DNA measurements made with a high-resolution scanning cytophotometer. Chromosomes were sorted from each peak by an electronic cell sorter. Visual examination of each fraction indicated the purity of the sorted chromosomes. This novel technology allows separation of purified populations of individual chromosomes suitable for biochemical and biological characterizations.
Proceedings of the National Academy of Sciences of the United States of America © 1975 National Academy of Sciences