If you need an accessible version of this item please contact JSTOR User Support

Chromosome Measurement and Sorting by Flow Systems

J. W. Gray, A. V. Carrano, L. L. Steinmetz, M. A. Van Dilla, D. H. Moore, B. H. Mayall and M. L. Mendelsohn
Proceedings of the National Academy of Sciences of the United States of America
Vol. 72, No. 4 (Apr., 1975), pp. 1231-1234
Stable URL: http://www.jstor.org/stable/64823
Page Count: 4
  • Download PDF
  • Cite this Item

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If you need an accessible version of this item please contact JSTOR User Support
Chromosome Measurement and Sorting by Flow Systems
Preview not available

Abstract

A flow microfluorometer was used to measure metaphase chromosomes in suspension at rates up to 100,000 per min. Chromosomes from cells of the Chinese hamster M3-1 cell line were isolated, stained for DNA with the fluorescent dye ethidium bromide, and analyzed for DNA content. Nine distinct peaks were resolved that correspond well with independent chromosomal DNA measurements made with a high-resolution scanning cytophotometer. Chromosomes were sorted from each peak by an electronic cell sorter. Visual examination of each fraction indicated the purity of the sorted chromosomes. This novel technology allows separation of purified populations of individual chromosomes suitable for biochemical and biological characterizations.

Page Thumbnails

  • Thumbnail: Page 
1231
    1231
  • Thumbnail: Page 
1232
    1232
  • Thumbnail: Page 
1233
    1233
  • Thumbnail: Page 
1234
    1234