If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Journal Article

Induction of Maturation (Meiosis) in Small Xenopus laevis Oocytes by Injection of Maturation Promoting Factor

J. Hanocq-Quertier, E. Baltus and J. Brachet
Proceedings of the National Academy of Sciences of the United States of America
Vol. 73, No. 6 (Jun., 1976), pp. 2028-2032
Stable URL: https://www.jstor.org/stable/65611
Page Count: 5
Were these topics helpful?

Select the topics that are inaccurate.

  • Read Online (Free)
  • Subscribe ($19.50)
  • Add to My Lists
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Induction of Maturation (Meiosis) in Small Xenopus laevis Oocytes by Injection of Maturation Promoting Factor
Preview not available

Abstract

Treatment of small- (stage III) or medium-sized (stage IV) Xenopus laevis oocytes with progesterone, human chorionic gonadotropin, or para-hydroxymercuriphenylsulfonate does not induce maturation. Only the full-grown oocytes (stage VI) undergo maturation when treated with either one of these three substances. In contrast, injection of maturation promoting factor into oocytes of stages III-VI invariably leads to chromosome condensation and germinal vesicle breakdown. No maturation spindle is found in oocytes smaller than 0.9 mm in diameter, and the nuclear sap does not mix with the cytoplasm in the smallest (0.45-0.55 mm in diameter) oocytes. In oocytes of 0.9 mm, maturation is identical to that of full-grown oocytes, except that the maturation spindle does not reach the cortex of the oocyte. Progesterone increases protein synthesis in medium-sized (0.8 mm in diameter) oocytes without inducing meiosis. It has little or no effect on protein synthesis in smaller oocytes.

Page Thumbnails

  • Thumbnail: Page 
2028
    2028
  • Thumbnail: Page 
2029
    2029
  • Thumbnail: Page 
2030
    2030
  • Thumbnail: Page 
2031
    2031
  • Thumbnail: Page 
2032
    2032